organisms" still deserves the careful attention. of bacteriologists, and confidently recommend this book to their notice.

ADRIAN J. BROWN.

SCHOOL OF MALTING AND BREWING, THE UNIVERSITY, BirminghAM, 16th October, 1902.

THE translators desire to express their best thanks to Mr. T. H. Pope for his great assistance in reading the manuscript and proofs of this translation during its progress through the press.

CONTENTS.

SECTION I. INTRODUCTION. (Pp. 1-15.)

SECTION II. THE LABORATORY. (Pp. 16-169.)

I. FITTINGS AND APPARATUS—

1. General Principles for fitting up the Laboratory

Preparation of Bench Surfaces

Solutions for Washing the Bench during Work

The Microscope Table

The Sterile Room

2. Hansen's Sterile Cupboard

3. The Microscope and its Accessories

Spherical and Chromatic Aberration

Achromatic and Apochromatic Objectives

The Tube

Correction Objectives

The Condenser

Immersion Objectives

The Stage

Changing the Objectives

Testing the Microscope .

Slips and Cover Glasses.

The Micrometer

Counting Apparatus

Klönne and Müller's Object Marker

Cover-Glass Gauge.

Apparatus for Artificial Illumination

Small Auxiliary Apparatus

Bottles for Reagents and Immersion Oil

4. Thermostats and their Accessories

Rohrbeck's Thermostat.

Panum's Thermostat

PAGE

Reichert's Regulator

Schribaux's Thermostat

Large Warm Chamber

A. Petersen's Thermostat for Low Temperatures.

Pfeiffer's Microscope Heating Apparatus

Muencke's Form of Lothar Meyer's Regulator

42

43

44

45

46

48

7. Apparatus for Spore Cultivation

Gypsum Blocks and their Containing Vessels

64

64

Solid Culture Media .

The Manipulation of Nutrient Liquids.

Experiments with Solid Culture Media

Cultures of Anaërobic Organisms.

Suppression of Bacteria in Yeast Growths

3. Preparation of Pure Cultures

Brefeld's Glass Slip Cultures

Hansen's Dilution Method

Dilution in and on Solid Substrata

Koch's Plate Culture

Surface Plate Cultures

1.06

Hansen's Saccharose Method for the Preservation of Yeasts

and Moulds

114

Preservation on Cotton Wool or Filter Paper (after Hansen) 117

Preservation of Bacteria

117

7. Counting of Yeast Cells and Seeding with a Definite Number

of Cells

126