Imaging in Neuroscience and Development: A Laboratory Manual

Portada
Rafael Yuste, Arthur Konnerth
CSHL Press, 2005 - 854 páginas
As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and well–received Imaging Neurons: A Laboratory Manualhas expanded to include development, with over twenty new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques.
 

Contenido

Ch 1 Maintaining Live Cells and Tissue Slices in the Imaging Setup
1
Ch 2 Video Microscopy Video Cameras and Image Enhancement
9
Ch 3 The Application of Scientificgrade CCD Cameras to Biological Imaging
23
Differential Interference Contrast Imaging of Living Cells
33
Infrared Video Microscopy
37
Principles and Practice
43
Ch 7 Principles of Multiphotonexcitation Fluorescence Microscopy
53
Ch 8 TwoPhoton Microscopy for 4D Imaging of Living Neurons
59
Imaging Microglia in Live Brain Slices and Slice Cultures
425
Ch 56 Single and Multiphoton Fluorescence Recovery after Photobleaching
429
Ch 57 AllOptical In Situ Histology of Neuronal Tissue with Ultrashort Laser Pulses
439
Spike Signals Population Signals and Retrograde Transport
445
Ch 59 Dendritic Voltage Imaging
457
Ch 60 Second Harmonic Imaging of Membrane Potential
463
Ch 61 Imaging Synaptic Vesicle Dynamics with Styryl Dyes
475
Imaging FM Dyes in Brain Slices
487

Building a TwoPhoton Laserscanning Microscope
71
How to Build a TwoPhoton Microscope Using a Confocal Scan Head
75
Ch 11 In Vivo Electroporation during Embryogenesis
79
In Vivo Electroporation of Neurons
95
SingleNeuron Labeling Using Genetic Methods
99
Ballistic Delivery of Dyes for Structural and Functional Studies of the Nervous System
111
Imaging Embryonic Development in Caenorhabditis elegans
119
Ch 16 Visualizing Morphogenesis in Frog Embryos
125
Ch 17 In Vivo Imaging of Synaptogenesis in the Embryonic Zebrafish
137
In Ovo Imaging of Avian Embryogenesis
149
Timelapse Imaging of the Formation of the Chick Peripheral Nervous System
153
Ch 20 Imaging Mouse Embryonic Development
159
Ch 21 Imaging the Developing Retina
171
Longterm TwoPhoton Transcranial Imaging of Synaptic Structures in the Living Brain
185
Ch 23 In Vivo Timelapse Imaging of Neuronal Development
191
Imaging 3D Organ Shapes and Gene Expression Patterns in Whole Vertebrate Embryos
205
Ch 25 Imaging the Development of the Neuromuscular Junction
215
Imaging Synaptogenesis by Measuring Accumulation of Synaptic Proteins
225
Imaging Retinotectal Synaptic Connectivity
229
Intrinsic Optical Imaging of Functional Map Development in Mammalian Visual Cortex
233
Ch 29 How Calcium Indicators Work
239
Ch 30 Some Quantitative Aspects of Calcium Fluorimetry
245
Ch 31 Calibration of Fluorescent Calcium Indicators
253
Ch 32 A SingleCompartment Model of Calcium Dynamics in Nerve Terminals and Dendrites
265
Dye Loading with Patch Pipettes
277
Calcium Imaging of the Retina
283
Ch 35 Calcium Imaging of Identified Astrocytes in Hippocampal Slices
289
Ch 36 Imaging Microscopic Calcium Signals in Excitable Cells
299
Ch 37 Monitoring Presynaptic Calcium Dynamics with Membranepermeant Indicators
307
TwoPhoton Calcium Imaging of Spines and Dendrites
315
Measurement of Free Ca2+ Concentration in the Lumen of Neuronal Endoplasmic Reticulum
319
Ch 40 Imaging Intracellular Calciumconcentration Microdomains at a Chemical Synapse
325
Ch 41 Monitoring Intramitochondrial Calcium with Rhod2
331
Ch 42 Generation of Controlled Calcium Oscillations in Nonexcitable Cells
341
HighSpeed Imaging of Calcium Waves in Neurons in Brain Slices
347
Imaging Action Potentials with Calcium Indicators
351
Ch 45 Imaging Calcium Transients in Developing Xenopus Spinal Neurons
357
Ch 46 Basics of Photoactivation
367
Ch 47 TwoPhoton Uncaging Microscopy
375
Chemical TwoPhoton Uncaging
385
Uncaging with Visible Light Inorganic Caged Compounds
391
Infraredguided Laser Stimulation of Neurons in Brain Slices
395
Ch 51 Uncaging Calcium in Neurons
399
Building a Simple Uncaging System
409
Ch 53 Ca2+ Uncaging in Nerve Terminals
415
Ch 54 Direct Multiphoton Stimulation of Neurons and Spines
421
Imaging Zinc in Brain Slices
491
Imaging Exocytosis with Total Internal Reflection Microscopy
495
Measuring Lightscattering Changes Associated with Secretion from Nerve Terminals
503
Ch 66 Imaging with Quantum Dots
511
Ch 67 Imaging Single Receptors with Quantum Dots
517
Tracking Receptors by Imaging Single Molecules
521
Imaging Sodium in Dendrites
527
TwoPhoton Sodium Imaging in Dendritic Spines
531
Ch 71 TwoPhoton Imaging of Chloride
535
Interferometric Detection of Action Potentials
539
Intrinsic Optical Signal Imaging in Brain Slices
545
Ch 74 Indicators Based on Fluorescence Resonance Energy Transfer
549
Ch 75 Cellular Imaging of Bioluminescence
557
Ch 76 Introduction of Green Fluorescent Protein into Hippocampal Neurons through Viral Infection
565
Ch 77 Green Fluorescent Proteins for Measuring Voltage
573
Ch 78 Genetic Probes for Calcium Dynamics
579
Targeted Recombinant Aequorins
589
Imaging Synaptic Inhibition with Clomeleon a Genetically Encoded Chloride Indicator
595
SynaptopHluorinsGenetically Encoded Reporters of Synaptic Transmission
599
Ch 82 Imaging Gene Expression in Live Cells and Tissues
605
Ch 83 Imaging Olfactory Activity in Drosophila CNS with a Calciumsensitive Green Fluorescent Protein
619
Ch 84 Tracking Molecules in Intact Zebrafish
623
Ch 85 Longterm HighResolution Imaging of Neurons in the Neocortex In Vivo
627
Ch 86 A TwoPhoton Fiberscope for Imaging in Freely Moving Animals
639
In Vivo TwoPhoton Calcium Imaging Using Multicell Bolus Loading
645
In Vivo Calcium Imaging in the Fly Visual System
649
Implications for Hemodynamicbased Functional Imaging
655
Ch 90 Voltagesensitive Dye Imaging of Neocortical Activity
673
WholeCell Recording and Voltagesensitive Dye Imaging In Vivo
689
In Vivo Imaging of Tumors
695
Ch 93 TwoPhoton Imaging of Cortical Microcirculation
701
Ch 94 Imaging Neuronal Activity with Calcium Indicators in Larval Zebrafish
707
Ch 95 Microscopy and Microscope Optical Systems
711
Ch 96 Practical Limits to Resolution in Fluorescence Light Microscopy
767
Ch 97 Lasers for Multiphoton Microscopy
775
Ch 98 Acoustooptic Tunable Filters for Microscopy
783
Arc Lamps and Monochromators for Fluorescence Microscopy
791
Ch 100 The Use of LiquidCrystal Tunable Filters for Fluorescence Imaging
797
Ch 101 Fluorescence Grating Imager Systems for Opticalsectioning Microscopy
805
Ch 102 Analysis of Dynamic Optical Imaging Data
815
Appendix 1 Electromagnetic Spectrum
827
Lenses Filters and EmissionExcitation Spectra
829
Appendix 3 Cautions
837
Imaging in Neuroscience and Development Index
843
Back cover
855
Derechos de autor

Otras ediciones - Ver todas

Imaging in Neuroscience and Development: A Laboratory Manual
Rafael Yuste,Arthur Konnerth
Vista de fragmentos - 2005
Imaging in Neuroscience and Development: A Laboratory Manual
Rafael Yuste,Arthur Konnerth
Sin vista previa disponible - 2005

Términos y frases comunes

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Información bibliográfica

TítuloImaging in Neuroscience and Development: A Laboratory Manual
EditoresRafael Yuste, Arthur Konnerth
Ediciónilustrada
EditorCSHL Press, 2005
ISBN0879696923, 9780879696924
Largo854 páginas
  
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