Imaging in Neuroscience and Development: A Laboratory ManualRafael Yuste, Arthur Konnerth CSHL Press, 2005 - 854 páginas As imaging studies have continued to expand in scope and sophistication, this new edition of the highly successful and well–received Imaging Neurons: A Laboratory Manualhas expanded to include development, with over twenty new chapters on such topics as MRI microscopy, imaging early developmental events, and labeling single neurons. Chapters on FRET, FCS/ICS, FRAP, hyperresolution microscopy, single molecule imaging, imaging with quantum dots, and imaging gene expression are included. With over forty full chapters, the manual also includes over forty sections of protocols for imaging techniques. |
Contenido
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23 | |
33 | |
37 | |
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59 | |
Imaging Microglia in Live Brain Slices and Slice Cultures | 425 |
Ch 56 Single and Multiphoton Fluorescence Recovery after Photobleaching | 429 |
Ch 57 AllOptical In Situ Histology of Neuronal Tissue with Ultrashort Laser Pulses | 439 |
Spike Signals Population Signals and Retrograde Transport | 445 |
Ch 59 Dendritic Voltage Imaging | 457 |
Ch 60 Second Harmonic Imaging of Membrane Potential | 463 |
Ch 61 Imaging Synaptic Vesicle Dynamics with Styryl Dyes | 475 |
Imaging FM Dyes in Brain Slices | 487 |
71 | |
75 | |
79 | |
In Vivo Electroporation of Neurons | 95 |
SingleNeuron Labeling Using Genetic Methods | 99 |
Ballistic Delivery of Dyes for Structural and Functional Studies of the Nervous System | 111 |
Imaging Embryonic Development in Caenorhabditis elegans | 119 |
Ch 16 Visualizing Morphogenesis in Frog Embryos | 125 |
Ch 17 In Vivo Imaging of Synaptogenesis in the Embryonic Zebrafish | 137 |
In Ovo Imaging of Avian Embryogenesis | 149 |
Timelapse Imaging of the Formation of the Chick Peripheral Nervous System | 153 |
Ch 20 Imaging Mouse Embryonic Development | 159 |
Ch 21 Imaging the Developing Retina | 171 |
Longterm TwoPhoton Transcranial Imaging of Synaptic Structures in the Living Brain | 185 |
Ch 23 In Vivo Timelapse Imaging of Neuronal Development | 191 |
Imaging 3D Organ Shapes and Gene Expression Patterns in Whole Vertebrate Embryos | 205 |
Ch 25 Imaging the Development of the Neuromuscular Junction | 215 |
Imaging Synaptogenesis by Measuring Accumulation of Synaptic Proteins | 225 |
Imaging Retinotectal Synaptic Connectivity | 229 |
Intrinsic Optical Imaging of Functional Map Development in Mammalian Visual Cortex | 233 |
Ch 29 How Calcium Indicators Work | 239 |
Ch 30 Some Quantitative Aspects of Calcium Fluorimetry | 245 |
Ch 31 Calibration of Fluorescent Calcium Indicators | 253 |
Ch 32 A SingleCompartment Model of Calcium Dynamics in Nerve Terminals and Dendrites | 265 |
Dye Loading with Patch Pipettes | 277 |
Calcium Imaging of the Retina | 283 |
Ch 35 Calcium Imaging of Identified Astrocytes in Hippocampal Slices | 289 |
Ch 36 Imaging Microscopic Calcium Signals in Excitable Cells | 299 |
Ch 37 Monitoring Presynaptic Calcium Dynamics with Membranepermeant Indicators | 307 |
TwoPhoton Calcium Imaging of Spines and Dendrites | 315 |
Measurement of Free Ca2+ Concentration in the Lumen of Neuronal Endoplasmic Reticulum | 319 |
Ch 40 Imaging Intracellular Calciumconcentration Microdomains at a Chemical Synapse | 325 |
Ch 41 Monitoring Intramitochondrial Calcium with Rhod2 | 331 |
Ch 42 Generation of Controlled Calcium Oscillations in Nonexcitable Cells | 341 |
HighSpeed Imaging of Calcium Waves in Neurons in Brain Slices | 347 |
Imaging Action Potentials with Calcium Indicators | 351 |
Ch 45 Imaging Calcium Transients in Developing Xenopus Spinal Neurons | 357 |
Ch 46 Basics of Photoactivation | 367 |
Ch 47 TwoPhoton Uncaging Microscopy | 375 |
Chemical TwoPhoton Uncaging | 385 |
Uncaging with Visible Light Inorganic Caged Compounds | 391 |
Infraredguided Laser Stimulation of Neurons in Brain Slices | 395 |
Ch 51 Uncaging Calcium in Neurons | 399 |
Building a Simple Uncaging System | 409 |
Ch 53 Ca2+ Uncaging in Nerve Terminals | 415 |
Ch 54 Direct Multiphoton Stimulation of Neurons and Spines | 421 |
Imaging Zinc in Brain Slices | 491 |
Imaging Exocytosis with Total Internal Reflection Microscopy | 495 |
Measuring Lightscattering Changes Associated with Secretion from Nerve Terminals | 503 |
Ch 66 Imaging with Quantum Dots | 511 |
Ch 67 Imaging Single Receptors with Quantum Dots | 517 |
Tracking Receptors by Imaging Single Molecules | 521 |
Imaging Sodium in Dendrites | 527 |
TwoPhoton Sodium Imaging in Dendritic Spines | 531 |
Ch 71 TwoPhoton Imaging of Chloride | 535 |
Interferometric Detection of Action Potentials | 539 |
Intrinsic Optical Signal Imaging in Brain Slices | 545 |
Ch 74 Indicators Based on Fluorescence Resonance Energy Transfer | 549 |
Ch 75 Cellular Imaging of Bioluminescence | 557 |
Ch 76 Introduction of Green Fluorescent Protein into Hippocampal Neurons through Viral Infection | 565 |
Ch 77 Green Fluorescent Proteins for Measuring Voltage | 573 |
Ch 78 Genetic Probes for Calcium Dynamics | 579 |
Targeted Recombinant Aequorins | 589 |
Imaging Synaptic Inhibition with Clomeleon a Genetically Encoded Chloride Indicator | 595 |
SynaptopHluorinsGenetically Encoded Reporters of Synaptic Transmission | 599 |
Ch 82 Imaging Gene Expression in Live Cells and Tissues | 605 |
Ch 83 Imaging Olfactory Activity in Drosophila CNS with a Calciumsensitive Green Fluorescent Protein | 619 |
Ch 84 Tracking Molecules in Intact Zebrafish | 623 |
Ch 85 Longterm HighResolution Imaging of Neurons in the Neocortex In Vivo | 627 |
Ch 86 A TwoPhoton Fiberscope for Imaging in Freely Moving Animals | 639 |
In Vivo TwoPhoton Calcium Imaging Using Multicell Bolus Loading | 645 |
In Vivo Calcium Imaging in the Fly Visual System | 649 |
Implications for Hemodynamicbased Functional Imaging | 655 |
Ch 90 Voltagesensitive Dye Imaging of Neocortical Activity | 673 |
WholeCell Recording and Voltagesensitive Dye Imaging In Vivo | 689 |
In Vivo Imaging of Tumors | 695 |
Ch 93 TwoPhoton Imaging of Cortical Microcirculation | 701 |
Ch 94 Imaging Neuronal Activity with Calcium Indicators in Larval Zebrafish | 707 |
Ch 95 Microscopy and Microscope Optical Systems | 711 |
Ch 96 Practical Limits to Resolution in Fluorescence Light Microscopy | 767 |
Ch 97 Lasers for Multiphoton Microscopy | 775 |
Ch 98 Acoustooptic Tunable Filters for Microscopy | 783 |
Arc Lamps and Monochromators for Fluorescence Microscopy | 791 |
Ch 100 The Use of LiquidCrystal Tunable Filters for Fluorescence Imaging | 797 |
Ch 101 Fluorescence Grating Imager Systems for Opticalsectioning Microscopy | 805 |
Ch 102 Analysis of Dynamic Optical Imaging Data | 815 |
Appendix 1 Electromagnetic Spectrum | 827 |
Lenses Filters and EmissionExcitation Spectra | 829 |
Appendix 3 Cautions | 837 |
Imaging in Neuroscience and Development Index | 843 |
Back cover | 855 |
Otras ediciones - Ver todas
Imaging in Neuroscience and Development: A Laboratory Manual Rafael Yuste,Arthur Konnerth Vista de fragmentos - 2005 |
Imaging in Neuroscience and Development: A Laboratory Manual Rafael Yuste,Arthur Konnerth Sin vista previa disponible - 2005 |
Términos y frases comunes
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Referencias a este libro
Confocal Microscopy and Multiphoton Excitation Microscopy: The Genesis of ... Barry R. Masters Sin vista previa disponible - 2006 |
Confocal Microscopy and Multiphoton Excitation Microscopy: The Genesis of ... Barry R. Masters Vista previa limitada - 2006 |