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pensable adjuncts, and by their means new substances have been prepared and fresh contributions made to the science of chemistry.

Momont is not able to give any satisfactory explanation of this different behaviour of the anthrax bacilli in these two media, but goes on to show that yet another factor plays an important part during insolation.

In the above experiments air was allowed to gain access to the vessels containing the broth, but if the precaution be taken of first removing the air and then exposing them to the sunshine, a very different result was obtained, for instead of the anthrax bacilli dying in from two to two and a half hours, they were found to be still alive after fifty hours' insolation. There appears, therefore, to be no doubt that sunshine in some way or other endows atmospheric oxygen with destructive power over the living protoplasm of the bacterial cells; indeed, there is considerable reason to believe that the bactericidal effect is due to the generation of peroxide of hydrogen, which is well known to possess powerfully antiseptic properties.

Numerous investigations have been also made to determine whether all the rays of the spectrum are equally responsible for the bactericidal action of light.

Geisler's work in St. Petersburg is especially instructive in this respect, for by decomposing with a prism the sun's light, as well as that emitted by a 1,000-candle-power electric lamp into their constituent rays, he was able to compare the different effects produced by the separate individual rays of both these sources of light.

The organism selected was the typhoid bacillus, and it was found that its growth was retarded in all parts of the two spectra excepting in the red, and that the intensity of the retardation was increased in passing from the red towards the ultraviolet end of the spectrum, where it was most pronounced of all.

But whereas from two to three hours of sunshine were sufficient to produce a most markedly deleterious effect upon the typhoid bacillus, a similar result was only obtained by six hours' exposure to the electric light.

Dr. Kirstein, of the University of Giessen, in the course of some experiments he made to ascertain how long different varieties of bacteria can exist when they obtain access to the air in the form of fine spray, and subsequently, as happens under ordinary circumstances, get dried up, noted also the effect upon their vitality of exposure in daylight and darkness respectively. For this purpose the apparatus in which the experiments

were carried out was in some cases kept in a dark cellar, whilst in others it was left standing in the laboratory in ordinary daylight.

Delicate bacteria, such as the fowl-cholera bacillus, it was found, could not survive exposure to daylight in this dried-up condition for more than ten hours, but when they were put in the dark their lease of life was prolonged for more than twice that length of time; whilst as regards varieties of tougher constitution, such as diphtheria and tubercle bacilli, whose initial vitality was very considerably greater under these adverse circumstances, confinement in the cellar enabled them to exist more than four times as long as they were able to in the healthy atmosphere of the well-lighted laboratory.

Dr. Onorato, of the University of Genoa, has recently shown, also, that influenza bacilli are entirely destroyed after the sun has been shining on them continuously for three and a half hours.

Such facts indicate how essential to health is plenty of light in our dwelling-rooms, and how important it is that in the designing of houses the trapping of the maximum amount of sunshine should be very carefully considered. Architects might indeed with advantage be compelled to include in their qualifications a knowledge of the fundamental facts of sanitary science. The

fashion of shutting the sunshine out by barriers of blinds and curtains drawn across the windows, a practice which seems to be almost entirely independent of the habitual gloom of the surroundings or general scarcity of sunshine, might possibly be modified were it but known that by thus excluding light we are conferring an inestimable benefit upon the members of the microbial community, which may at any moment comprise some of the subtlest and most dangerous antagonists with which we have to reckon in the struggle for existence.

From a hygienic point of view, also, the question of the potency of sunshine in regard to the bacteria present in water is both important and interesting, for it is to water at the present time that we look for the dissemination of some of the most dreaded zymotic diseases.

Comparatively little has been done in this direction, but those results which have been obtained are exceedingly suggestive. Professor Buchner has published some preliminary experiments which he made with particular micro-organisms. In these investigations boiled tap-water was used to ensure the absence of all bacteria except those which were subsequently introduced, and, whilst some of the vessels were exposed to the sunshine, others were simultaneously preserved

in the dark. It was found that typhoid, cholera, and various other bacilli were most deleteriously affected by insolation. Perhaps an example will best serve to illustrate the nature of the results obtained. Some boiled water contained in a flask was inoculated with an immense number of a bacillus, closely resembling the typhoid organism, normally present in the body and frequently found in water, the bacillus coli communis. So many were introduced that nearly one hundred thousand individuals were present in every twenty drops of the water. This flask then, containing water so densely sown with microbes, was placed in the sunshine for one hour, whilst another and similar flask was kept during the same time in the dark. On being subsequently examined it was ascertained that whereas a slight increase in the number of bacilli had taken place in the "dark" flask, in the insolated flask absolutely no living organisms whatever were present.

Professor Percy Frankland has also investigated the action of sunshine on micro-organisms in water, and in one of his reports to the Water Research Committee of the Royal Society an account is given of the effect of insolation on the vitality of the spores of anthrax in Thames water. These experiments show again what an important influence the surroundings of the organism

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